FastQCFastQC Report
Thu 2 Jun 2016
DRR015240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9641253
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300240.3114118050838413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251720.2610863961354401No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160010.16596390531396696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176000.053.33241
GTATCAA334500.041.2946661
ATCAACG420600.032.360613
TCAACGC428050.031.746344
TATCAAC439700.031.0427532
CAACGCA445300.030.5214755
AACGCAG465750.029.242266
GTGGTAT79250.028.5318451
TGGTATC80800.027.0557672
ACGCAGA525950.025.6709797
CGCAGAG525050.025.5329138
GTACATG362400.023.97631
GCAGAGT567650.023.119579
TACATGG352650.022.9882582
ACATGGG362600.021.411973
TACCTGG73050.020.3871972
AGAGTAC527050.020.21062710-11
GTACCGG10400.020.163781
GAGTACT360750.020.03783812-13
CAGAGTA556200.019.5565310-11
Produced by FastQC (version 0.11.5)