FastQCFastQC Report
Thu 2 Jun 2016
DRR015239_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015239_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5789872
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332600.5744513868354948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277550.4793715646908947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170330.29418612363105784No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84510.14596177601162857No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67610.11677287511710104No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156150.055.4274441
GTATCAA287250.039.5493051
TATAACG5550.038.535112
TACCTGG43950.032.8210222
GTGGTAT59000.031.0092931
ATCAACG365150.030.9892543
TGGTATC58300.030.6743762
TCAACGC371000.030.4880814
TATCAAC380700.029.8460872
CAACGCA389000.029.1922665
GTACATG317750.028.5163041
TACATGG310150.028.052262
AACGCAG407600.028.0035446
GTACCTG54650.027.4666231
ACATGGG324600.026.0017193
CGCAGAG446500.025.1432488
ACGCAGA450050.025.0578337
ACCTGGG57750.024.8179113
GCAGAGT466200.023.6217849
GTATAAC14050.023.0558741
Produced by FastQC (version 0.11.5)