FastQCFastQC Report
Thu 2 Jun 2016
DRR015239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5789872
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393670.6799286754525834No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318610.5502885037873031No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210400.36339318036737256No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85690.14799981761254827No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83610.14440733750245258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76030.13131551094739227No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61190.10568454708497874No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60410.10433736704369284No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166700.057.2630771
GTATCAA316800.041.9456561
TACCTGG47550.034.3858032
GTGGTAT61000.034.15231
ATCAACG400450.032.8412973
TCAACGC405950.032.4095274
TGGTATC63450.032.2292332
TATCAAC410650.032.1192
CAACGCA420200.031.4147175
TATAACG5800.031.4099482
AACGCAG439500.030.130226
ACCTGGG54750.028.944533
GTACCTG57800.028.7082791
GTACATG328050.027.1669161
GTACCGG5950.026.8154241
TACATGG320650.026.7173162
CGCAGAG490000.026.6912128
ACGCAGA493950.026.634447
ACATGGG328000.025.2262363
GCAGAGT513250.025.0652459
Produced by FastQC (version 0.11.5)