FastQCFastQC Report
Thu 2 Jun 2016
DRR015238_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015238_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3883304
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125450.3230496505037978No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108960.28058581043358954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64100.16506562453003937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74900.048.2470131
GTATCAA131300.037.5306551
ATCAACG159600.030.5077863
TCAACGC161600.030.1568164
TATCAAC166900.029.230962
CAACGCA168500.028.9759185
AACGCAG174100.028.2511626
TACCTGG29950.027.6553542
TCTGTCG11200.027.620958
GTACCTG33950.025.4009021
ACGCAGA193550.025.2926797
CGCAGAG196450.024.8250718
TAACGCA3850.024.8196664
GCAGAGT207400.022.9431929
GTACATG94050.022.3650171
CTGTCGC14600.021.4994419
ATAACGC4300.021.1651523
GTGGTAT37150.021.002181
GAGTACT147900.020.94885412-13
CGCTCTG11150.020.8129045
Produced by FastQC (version 0.11.5)