FastQCFastQC Report
Thu 2 Jun 2016
DRR015238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3883304
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153960.3964665140818231No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129770.3341741980540282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81270.20928055078870983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88700.048.9872781
GTATCAA148900.034.785451
TATAACG3800.031.165772
ATAACGC4350.030.361563
ATCAACG181600.028.1630213
TCAACGC183550.027.890434
TATCAAC186950.027.5809442
TACCTGG32550.027.2880022
TAACGCA4700.027.1333544
CAACGCA190350.026.9498795
AACGCAG198250.026.083616
TCTGTCG12250.025.6411768
GTACCTG35650.025.4511591
GTCTCGC11900.023.3720511
ACGCAGA220150.023.3660187
GTACATG93150.023.2500951
CGCAGAG222450.023.1448928
ACAACGC6350.022.2332673
GTGGTAT45800.022.1004681
TACATGG94450.021.6537192
Produced by FastQC (version 0.11.5)