FastQCFastQC Report
Thu 2 Jun 2016
DRR015237_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015237_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5724610
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147810.2582009953516484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136030.23762317432977964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75670.1321836771413249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99250.053.5518341
GTATCAA183000.039.3151131
ATCAACG225150.031.6667543
TCAACGC226450.031.5653324
TATCAAC233700.030.5692982
CAACGCA235600.030.3552865
AACGCAG247600.029.0298336
CGCAGAG272050.026.0174528
ACGCAGA274850.025.869167
GTGGTAT43250.025.632981
TGGTATC42900.024.0775952
GTACATG150850.024.043671
GCAGAGT293900.023.432719
TACATGG147850.022.559382
GAGTACT208550.020.5700712-13
ACATGGG157500.020.5686593
CAGAGTA292050.020.26474410-11
AGAGTAC272000.020.11952410-11
AGTACTT217400.018.88521612-13
GTACTTT227900.018.79386114-15
Produced by FastQC (version 0.11.5)