FastQCFastQC Report
Thu 2 Jun 2016
DRR015237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5724610
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165040.28829911557293864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150370.26267291570954177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85590.14951236852816174No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109950.051.6698151
GTATCAA194300.037.6161881
ATCAACG237050.030.448613
TCAACGC239100.030.2084454
CAACGCA248550.029.136485
TATCAAC249150.029.0843682
GTGGTAT51800.028.4306531
AACGCAG257850.028.174856
TGGTATC51900.026.6782042
ACGCAGA288800.024.8889967
CGCAGAG286400.024.8273568
GTACATG150000.023.9220031
GCAGAGT312650.022.4370969
GTACCGG4550.022.0457761
TACATGG148950.021.9244652
ACATGGG151600.020.6965623
GAGTACT231450.020.31692512-13
AGAGTAC288950.020.18128810-11
ACAACGC9050.020.127493
CAGAGTA313050.019.6964410-11
Produced by FastQC (version 0.11.5)