FastQCFastQC Report
Thu 2 Jun 2016
DRR015236_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015236_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5702272
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346460.6075823811982312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282300.4950658263934095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188540.3306401378257649No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98870.17338702888953736No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68150.11951376574109408No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162800.054.422741
GTATCAA307500.039.7478071
TACCTGG42800.038.3818472
TATAACG5900.031.6223622
ATCAACG393000.030.9718383
TCAACGC398450.030.5482044
ACCTGGG52800.030.4212153
GTACCTG55800.029.9248831
TATCAAC412200.029.6524432
CAACGCA416800.029.2220485
GTGGTAT64800.028.866521
TGGTATC62400.028.7325462
AACGCAG436750.027.9782876
GTACATG349350.027.1114141
TACATGG340750.026.6421642
ACGCAGA477000.025.3018137
CGCAGAG475850.025.2849248
TACAACG11050.024.7087862
ACATGGG361350.024.064033
GCAGAGT493900.023.7712069
Produced by FastQC (version 0.11.5)