FastQCFastQC Report
Thu 2 Jun 2016
DRR015236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5702272
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT431180.7561547397247974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339410.5952188881905318No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237300.41614991357830705No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97680.17130014141731575No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86620.15190436373431504No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84280.1478007362679297No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68080.1193910076544928No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66320.11630451861994658No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA183100.055.0487481
TACCTGG48800.043.116342
GTATCAA346000.040.3305631
TATAACG7650.039.887162
GTACCGG4500.037.4831661
ACCTGGG58200.035.050853
GTACCTG63300.034.568821
ATCAACG443900.031.0916883
TCAACGC450050.030.6583124
TAACGCA10750.030.4993364
TATCAAC459100.030.1568552
CAACGCA465500.029.7509545
TACAACG13300.029.448732
TGGTATC72150.029.2887882
GTGGTAT72750.028.825171
ACAACGC14100.028.741833
AACGCAG489850.028.4498356
GTACATG351800.025.8908731
CGCAGAG539550.025.4336418
ACGCAGA541250.025.4210327
Produced by FastQC (version 0.11.5)