FastQCFastQC Report
Thu 2 Jun 2016
DRR015235_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015235_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7782998
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190770.245111202649673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163090.20954650123255844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94480.12139281032835933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126800.052.4240571
GTATCAA224400.038.182391
ATCAACG268950.031.3013023
TCAACGC271900.030.9443634
CAACGCA285050.029.6420565
TATCAAC285550.029.6279352
AACGCAG295150.028.7174366
ACGCAGA332650.025.2189257
CGCAGAG335450.024.8448778
GTGGTAT55800.024.5288621
GTACATG211900.023.1240391
GCAGAGT363150.022.5237189
TGGTATC58350.021.8384252
TACATGG214050.021.2399942
TACCTGG57050.020.1024532
ACATGGG218450.020.0394253
CAGAGTA357750.019.87005810-11
GAGTACT247150.019.53971112-13
AGAGTAC338750.019.41993510-11
GTACCTG66350.018.634541
Produced by FastQC (version 0.11.5)