FastQCFastQC Report
Thu 2 Jun 2016
DRR015235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7782998
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224420.28834647008774766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194760.25023776184961116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119560.15361689672797038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133700.053.4234921
GTATCAA241800.038.3244361
ATCAACG296950.030.7134363
TCAACGC300150.030.40374
CAACGCA310100.029.4873525
TATCAAC314150.029.1684842
AACGCAG323350.028.323276
GTGGTAT61050.027.7748641
TGGTATC62800.025.092242
ACGCAGA367450.024.7639337
CGCAGAG369900.024.4030118
GTACATG210600.023.9381981
TACCTGG57900.022.8895572
GCAGAGT398800.022.314999
TATAACG7400.022.156122
TACATGG212100.021.9019322
ACAACGC11400.021.5684853
ACATGGG216600.020.4963683
GTACCTG66950.020.2209051
AGAGTAC375400.019.81357810-11
Produced by FastQC (version 0.11.5)