FastQCFastQC Report
Thu 2 Jun 2016
DRR015234_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015234_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6013632
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172970.28762983834062344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151700.25226019816310674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87490.145486122197035No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104650.055.976391
GTATCAA187000.038.5961461
ATCAACG225500.031.3748553
TCAACGC226950.031.1543524
TATCAAC233850.030.4723912
CAACGCA237650.029.8262425
GTGGTAT43200.029.3589251
AACGCAG247800.028.7313926
TGGTATC45350.025.9881062
ACGCAGA278600.025.4066147
CGCAGAG279850.025.162468
GTACATG149850.023.7171
GCAGAGT297850.023.1834459
TACCTGG44100.023.0100962
TACATGG151550.022.3092542
GTACCTG50300.021.8588921
TCTGTCG15800.021.017128
GAGTACT225550.020.90512-13
CAGAGTA298000.020.73011810-11
ACATGGG156400.020.5092743
Produced by FastQC (version 0.11.5)