FastQCFastQC Report
Thu 2 Jun 2016
DRR015234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6013632
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203220.33793221800070244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180970.30093294701105755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105030.17465318795696178No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107300.054.9734731
GTATCAA199250.040.334121
ATCAACG247900.032.0717963
GTGGTAT46600.031.9875131
TCAACGC250800.031.686754
TATCAAC257350.030.9338742
CAACGCA262100.030.356335
TGGTATC46450.029.5119442
AACGCAG272600.029.3562376
ATAACGC6150.026.6552623
TATAACG5950.026.0242962
ACGCAGA306550.025.853717
CGCAGAG306400.025.7772248
GTACATG140850.024.1111581
ACAACGC9650.024.0656973
GCAGAGT332200.023.4326789
TACATGG141500.022.9160962
TAACGCA8350.022.3618244
ACATGGG141400.021.7375183
TCTGTCG17850.020.9122668
Produced by FastQC (version 0.11.5)