FastQCFastQC Report
Thu 2 Jun 2016
DRR015233_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015233_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5608008
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169670.3025494970763237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140800.251069541983535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85980.15331647173113877No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95800.052.867781
GTATCAA174350.039.0986941
ATCAACG211800.031.688893
TCAACGC213850.031.256894
TATCAAC220250.030.662082
CAACGCA224800.029.7918995
GTGGTAT41350.029.3521351
AACGCAG235100.028.7176276
TGGTATC41850.027.5111482
TATAACG5250.026.8711362
CGCAGAG257400.025.7310838
ACGCAGA260350.025.685977
TACCTGG47650.025.0220012
GTACCTG49250.024.8291661
TCTGTCG18300.024.6087388
GTCTCGC16350.024.0001681
GTACATG124350.023.924091
ACAACGC10350.023.740753
CTGTCGC20400.023.6357469
GCAGAGT275700.023.511039
Produced by FastQC (version 0.11.5)