FastQCFastQC Report
Thu 2 Jun 2016
DRR015233_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015233_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5608008
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198660.3542434318923939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173820.30994962917313956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104770.18682213006828807No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103050.053.923251
GTATCAA192800.039.697581
ATCAACG235050.032.2362143
TCAACGC239650.031.562424
TATCAAC243150.031.259882
CAACGCA248050.030.6237775
ATAACGC5700.030.3570583
GTGGTAT45700.030.0241451
AACGCAG260250.029.4160676
TATAACG5950.028.3197882
TGGTATC46000.027.9187852
GTACCTG48000.026.9710241
TACCTGG47000.026.8402842
ACGCAGA288200.026.2323197
CGCAGAG287450.026.1582538
ACAACGC8900.024.558523
GTCTCGC15800.023.9464591
GCAGAGT309850.023.8411989
GTACATG130500.023.3338431
TACATGG134250.021.812322
Produced by FastQC (version 0.11.5)