FastQCFastQC Report
Thu 2 Jun 2016
DRR015232_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015232_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7504053
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196680.2620983620451508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161060.21463068024706114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104920.13981777580728708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150600.043.536631
GTATCAA260050.036.96841
ATCAACG316650.029.9057043
TCAACGC319750.029.5588384
TATCAAC330600.028.7567982
CAACGCA332600.028.3729465
AACGCAG344450.027.514266
ACGCAGA381650.024.45017
CGCAGAG379850.024.433238
GTACATG225800.023.884521
TACATGG221050.022.9762212
GCAGAGT408750.022.215549
ACATGGG231850.021.000913
GAGTACT254500.020.22833312-13
AGAGTAC360300.020.10284610-11
ACAACGC13350.019.088343
AGTACTT267400.019.0057512-13
GTACTTT274450.018.5419714-15
CAGAGTA408150.018.52078210-11
GTGGTAT86150.018.483881
Produced by FastQC (version 0.11.5)