FastQCFastQC Report
Thu 2 Jun 2016
DRR015232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7504053
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232200.3094327825243238No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194110.2586735461489944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126310.16832237192354585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172650.045.068041
GTATCAA297900.036.325531
ATCAACG366800.028.9628123
TCAACGC370900.028.5701314
TATCAAC381000.028.042472
CAACGCA383100.027.7179375
AACGCAG395700.026.9519796
ACGCAGA438650.024.1682077
CGCAGAG437400.024.0395478
GTACCGG6150.023.7179221
ACAACGC11100.022.5629043
GTACATG232450.022.3747331
GCAGAGT469150.022.0439649
TACATGG227550.021.5552042
AGAGTAC409600.020.97031810-11
GAGTACT298400.020.56247912-13
AGTACTT312650.020.24402812-13
ACATGGG231050.020.1615983
GTACCTG58750.020.095261
TACCTGG56350.019.6392822
Produced by FastQC (version 0.11.5)