FastQCFastQC Report
Thu 2 Jun 2016
DRR015231_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015231_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7429176
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208180.2802195021359031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174790.23527508299709146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103110.13879062765507238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119700.054.8354721
GTATCAA220100.039.8109251
ATCAACG272600.031.746613
TCAACGC274600.031.415554
TATCAAC286650.030.2556922
CAACGCA288400.029.9733925
AACGCAG304450.028.5708476
ACGCAGA340300.025.2651447
GTACATG234150.025.0519641
CGCAGAG342550.025.0190078
GTGGTAT50950.024.5302121
TACATGG231050.023.9870852
GCAGAGT367400.022.7199349
TGGTATC51700.022.707292
ACATGGG238850.022.2500153
GAGTACA177450.020.6411761
GAGTACT250600.020.12292912-13
CAGAGTA363450.019.79525610-11
AGAGTAC346400.019.67956210-11
TATAACG6700.019.0160082
Produced by FastQC (version 0.11.5)