FastQCFastQC Report
Thu 2 Jun 2016
DRR015231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7429176
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249040.3352188721871712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209720.2822924103561418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126400.17013999937543545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133550.055.6375581
GTATCAA247300.040.700461
ATCAACG307200.032.537033
TCAACGC308450.032.3487364
CAACGCA318350.031.3563675
TATCAAC321400.031.272882
GTGGTAT53900.030.192831
AACGCAG333000.030.0737766
TGGTATC58300.027.1064322
CGCAGAG378700.026.2174438
ACGCAGA379800.026.201447
GTACATG240700.025.1315421
GCAGAGT407550.024.0152749
TACATGG234950.023.9388312
ACATGGG240450.022.5743923
TATAACG5950.020.6660842
GAGTACT294100.020.58662412-13
CAGAGTA403550.020.49687210-11
AGAGTAC390550.020.07180810-11
GTACTTT312350.019.26505314-15
Produced by FastQC (version 0.11.5)