FastQCFastQC Report
Thu 2 Jun 2016
DRR015230_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015230_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5390270
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146370.271544839126797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124130.23028531038333885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74700.1385830394395828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88000.049.5666051
GTATCAA152500.038.3259771
TAACGCA5150.031.8068644
TACCTGG47550.031.1998982
ATCAACG186900.030.6516653
TCAACGC186900.030.553434
TATAACG4850.030.0257842
CAACGCA194700.029.466895
TATCAAC197250.029.1619262
AACGCAG202150.028.4465946
GTGGTAT37950.027.4117661
GTACCTG61900.024.987511
ACGCAGA231450.024.5490067
GTACATG174950.024.540851
ATAACGC6250.024.0254543
TACATGG176700.023.5394212
CGCAGAG241250.023.4561928
TGGTATC42900.023.3373472
ACCTGGG62600.023.2601933
ACATGGG179750.022.4539323
Produced by FastQC (version 0.11.5)