FastQCFastQC Report
Thu 2 Jun 2016
DRR015230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5390270
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179460.3329332296897929No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150510.279225344927063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94100.17457381541184394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92400.052.4564631
GTATCAA166050.039.7344671
ATCAACG205950.031.4894563
TCAACGC206850.031.3133584
CAACGCA213350.030.4878435
TACCTGG46800.030.3664722
TATCAAC216250.030.3524282
AACGCAG220750.029.5494046
GTGGTAT40600.027.2909051
GTACATG173550.025.4324651
ACGCAGA255350.025.3139387
ACAACGC9250.024.6175753
GTACCTG61750.024.441551
CGCAGAG262650.024.3850658
TATAACG4500.024.2931772
TACATGG173900.024.2547592
ACCTGGG57850.023.302653
ACATGGG175300.023.0960033
TGGTATC46300.022.922422
GCAGAGT284100.022.2555459
Produced by FastQC (version 0.11.5)