FastQCFastQC Report
Thu 2 Jun 2016
DRR015229_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015229_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8851346
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198700.22448563190276372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166380.18797141135370823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98410.11118083057650216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133850.049.8392641
GTATCAA246550.040.021921
ATCAACG303350.032.0176123
TCAACGC306900.031.6593994
CAACGCA321150.030.3784855
TATCAAC324550.030.1372952
AACGCAG335850.029.1261336
ACGCAGA376800.025.6932017
CGCAGAG375250.025.593328
GTACATG236850.024.6231061
GCAGAGT408400.022.9925829
TACATGG238850.022.8942622
GTGGTAT66650.021.6872651
ACATGGG247250.021.5270583
TGGTATC64650.020.7587342
AGAGTAC370600.020.55264510-11
GTACCTG71250.019.967121
GAGTACT268700.019.9457212-13
CAGAGTA401300.019.18430710-11
AGTACTT288400.018.65422812-13
Produced by FastQC (version 0.11.5)