FastQCFastQC Report
Thu 2 Jun 2016
DRR015229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8851346
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230560.2604801574811334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198290.2240224255158481No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117360.1325900038254069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151400.050.786851
GTATCAA272800.038.7410551
ATCAACG333700.031.1808243
TCAACGC338150.030.824534
TATCAAC351200.029.7475072
CAACGCA351900.029.6355925
AACGCAG366650.028.4559546
ACGCAGA415900.024.954647
CGCAGAG415000.024.8442258
GTACATG239550.023.9450841
TATAACG9200.023.7610192
TACATGG238100.022.5318872
GCAGAGT451950.022.4303059
GTGGTAT75350.022.3470571
TGGTATC72750.022.035422
ATAACGC10850.021.4041523
ACATGGG238600.021.394043
GTACCTG70700.020.589631
AGAGTAC414900.019.91845710-11
ACAACGC12150.019.8635653
Produced by FastQC (version 0.11.5)