FastQCFastQC Report
Thu 2 Jun 2016
DRR015228_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015228_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6577144
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172900.2628800585786171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153480.23335356501241267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86890.13210901266567981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115500.054.602871
GTATCAA210850.040.075331
ATCAACG261050.031.9039613
TCAACGC263300.031.5953374
TATCAAC270750.030.9310762
CAACGCA273400.030.6249775
AACGCAG284650.029.458066
GTGGTAT50900.028.1311281
TGGTATC49750.027.4307652
ACGCAGA316300.026.2202647
CGCAGAG317250.025.9556318
GCAGAGT340650.023.6786359
GTACATG183050.021.573641
GTACCTG52750.021.006761
ACAACGC11750.020.9043123
GAGTACT235200.020.79207212-13
CAGAGTA336850.020.49681910-11
TACCTGG51750.020.4811952
AGAGTAC320650.020.10727110-11
TACATGG187500.020.0395742
Produced by FastQC (version 0.11.5)