FastQCFastQC Report
Thu 2 Jun 2016
DRR015228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6577144
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206520.3139964702004396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181030.2752410468738407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104750.1592636560792952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121950.055.606161
GTATCAA223150.039.9998051
ATCAACG277500.031.6095813
TCAACGC280000.031.3763894
GTGGTAT52850.031.170811
TATCAAC287450.030.6428782
TGGTATC50750.030.3330462
CAACGCA291500.030.1576355
AACGCAG305250.028.81416
ACGCAGA344950.025.4051367
CGCAGAG343150.025.37928
GCAGAGT371250.023.1271539
TATAACG6350.022.9515292
ATAACGC7850.020.8861473
ACAACGC10050.020.3925673
GTACATG180350.020.3688071
GAGTACT265000.020.30534212-13
AGAGTAC349700.020.25618210-11
CAGAGTA367400.020.0795310-11
TACATGG180800.019.295932
Produced by FastQC (version 0.11.5)