FastQCFastQC Report
Thu 2 Jun 2016
DRR015227_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015227_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5969804
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182430.3055879221495379No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153280.2567588483642009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95860.16057478603987668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131900.039.068421
TACCTGG58900.034.21412
GTATCAA213500.033.961861
GTACCTG59600.033.360491
TATAACG6550.032.641742
ATAACGC6850.031.8746593
TAACGCA7950.028.0348574
ATCAACG256050.028.0157183
TCAACGC258200.027.7808064
TATCAAC264350.027.1718712
CAACGCA269850.026.6976475
AACGCAG280000.025.9204356
GTACATG144550.025.301631
TCTGTCG18650.024.6258818
GTACCGG6000.024.3215311
TACATGG145600.024.213472
ACAACGC12250.023.3937233
CGCAGAG309150.023.1370898
ACGCAGA309700.023.0959997
GTCTCGC19300.022.2107261
Produced by FastQC (version 0.11.5)