FastQCFastQC Report
Thu 2 Jun 2016
DRR015227_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015227_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5969804
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214260.3589062555487584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181540.3040970859344796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114530.1918488446186843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141800.042.203611
TATAACG5700.041.5541462
GTACCTG58850.036.766731
ACAACGC10850.036.1003073
TACCTGG59900.034.9797632
GTATCAA235800.034.729761
ATAACGC7750.031.1470093
TAACGCA8600.029.1277164
ATCAACG285150.028.4148083
TCAACGC288300.028.135944
TATCAAC292800.027.67522
TACAACG14200.027.586462
CAACGCA299250.027.2296815
AACGCAG311950.026.2818576
TCTGTCG19200.023.9471998
GTACATG144900.023.9424361
GTCTCGC18450.023.751881
ACCTGGG86300.023.6961463
GTACCGG4900.023.2898711
ACGCAGA348650.023.2545977
Produced by FastQC (version 0.11.5)