FastQCFastQC Report
Thu 2 Jun 2016
DRR015226_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015226_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8601054
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268110.31171761042309465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231220.268827518115803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137120.1594223219619363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA171700.051.4867481
GTATCAA299550.038.1112331
ATCAACG371450.030.5531623
TCAACGC376300.030.1704084
TATCAAC380600.029.855532
CAACGCA394800.028.766665
GTGGTAT70350.028.1263661
AACGCAG413800.027.6074736
ACGCAGA461650.024.320247
GTACATG336350.024.222621
CGCAGAG466600.023.8575768
TGGTATC80450.023.6913132
TACATGG340950.022.9343452
GCAGAGT492700.022.0215329
ACATGGG348650.021.4746513
TACCTGG68600.021.0864832
GAGTACT302250.020.19919812-13
CAGAGTA478550.019.8317510-11
AGAGTAC457350.019.19495410-11
GTACTTT317300.019.14807314-15
Produced by FastQC (version 0.11.5)