FastQCFastQC Report
Thu 2 Jun 2016
DRR015226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8601054
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324820.377651390166833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276100.3210071695864251No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175350.2038703628648303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176250.053.309931
GTATCAA326650.041.463131
ATCAACG417250.032.030523
TCAACGC420750.031.785724
TATCAAC428150.031.2937132
CAACGCA436750.030.5973155
AACGCAG455700.029.4847556
GTGGTAT74900.027.4773981
ACGCAGA518500.025.71177
CGCAGAG521550.025.4391638
TGGTATC79850.024.4106452
GCAGAGT554400.023.5459449
GTACATG346050.023.0638521
TACATGG345050.022.1868722
ACATGGG350700.020.8267173
AGAGTAC515300.020.60258510-11
GAGTACT352650.020.54799712-13
AGTACTT357200.020.34359712-13
CAGAGTA541250.019.93435710-11
GTACTTT367300.019.41095714-15
Produced by FastQC (version 0.11.5)