FastQCFastQC Report
Thu 2 Jun 2016
DRR015225_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015225_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7456648
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299910.4022048512951128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256740.34431020479979746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152470.20447525483300272No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75960.10186882899662153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162800.055.4558031
GTATCAA301700.040.51891
ATCAACG380150.032.068163
TCAACGC382800.031.8567624
TATCAAC390500.031.3003792
CAACGCA404650.030.112895
AACGCAG425400.028.7570346
GTGGTAT65800.026.9562741
GTACATG348750.025.6650581
ACGCAGA478100.025.3280017
CGCAGAG476700.025.3260758
TACATGG346250.025.1057912
TGGTATC67850.024.8058932
GCAGAGT501050.023.5235279
ACATGGG356500.023.388223
TATAACG8350.020.7014182
TACCTGG60650.020.4005132
GAGTACT317650.020.2791512-13
CAGAGTA493750.020.1925510-11
CATGGGG164750.019.9889454
Produced by FastQC (version 0.11.5)