FastQCFastQC Report
Thu 2 Jun 2016
DRR015225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7456648
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349470.46866903198327187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299650.4018561691526809No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185630.24894563884469267No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83620.11214154134672845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA174650.056.192431
GTATCAA326050.041.1124651
GTGGTAT68200.033.9848441
ATCAACG414350.031.9585193
TCAACGC418450.031.6780364
TATCAAC421850.031.5649532
TGGTATC72500.031.0295012
CAACGCA434750.030.5527845
AACGCAG454800.029.245896
ACGCAGA513400.025.7570067
CGCAGAG511900.025.6635238
GTACATG351150.025.3495711
TACATGG347050.024.8920762
GCAGAGT545600.023.7446429
ACATGGG354950.023.489883
TACCTGG60050.023.1298432
TATAACG7300.022.457712
GAGTACT356800.020.87218512-13
ATAACGC8400.020.5998443
GTACCTG70700.020.586251
Produced by FastQC (version 0.11.5)