FastQCFastQC Report
Thu 2 Jun 2016
DRR015224_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015224_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5818648
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223880.3847629208709652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194330.3339779275185576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116290.19985742392390812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118200.051.300991
GTATCAA208900.039.612271
TACCTGG57550.033.4345472
TATAACG6950.032.725452
ATCAACG262700.031.3768793
TCAACGC266100.031.009364
GTACCTG65050.030.8387661
TATCAAC270300.030.5275272
CAACGCA279800.029.5540285
AACGCAG293050.028.337556
GTACATG228050.026.2098311
ACCTGGG73750.026.1526363
GTGGTAT49700.026.1444841
TACATGG227750.025.1659072
ACAACGC15050.025.087263
TAACGCA8550.025.0053064
ACGCAGA329350.024.9368157
CGCAGAG330750.024.7214788
ATAACGC9150.024.3605233
TACAACG14850.024.1992022
Produced by FastQC (version 0.11.5)