FastQCFastQC Report
Thu 2 Jun 2016
DRR015224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5818648
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269880.4638190864956945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223560.38421296493618445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145510.2500752752185731No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62740.10782573546294603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131750.053.200291
GTATCAA238200.040.40251
TACCTGG56050.034.4505232
ATCAACG295950.032.204413
TCAACGC299200.031.8700944
TATCAAC307500.031.234682
CAACGCA309900.030.8328325
GTGGTAT56100.030.8283161
GTACCTG65350.030.7940251
ACAACGC11650.030.0974253
AACGCAG322900.029.7888666
TGGTATC56950.029.0280932
ACCTGGG68950.026.7476273
GTACCGG6150.026.7116571
TATAACG6050.026.3462182
ACGCAGA369200.025.8186657
CGCAGAG371700.025.4980498
TACAACG12350.025.444142
GTACATG228250.024.5705321
TACATGG228600.023.5874562
Produced by FastQC (version 0.11.5)