FastQCFastQC Report
Thu 2 Jun 2016
DRR015223_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015223_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7622512
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207370.2720494241268495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185410.24324002376119577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105940.13898305440516198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130550.051.0621
GTATCAA234550.039.75441
ATCAACG287500.031.9912553
TCAACGC288750.031.8032074
CAACGCA300400.030.5369385
TATCAAC302650.030.3910392
AACGCAG314250.029.2590226
ACGCAGA351800.025.8882087
CGCAGAG352450.025.6595158
GTACATG221800.023.8670061
GCAGAGT381700.023.0261279
TACATGG225900.021.8684842
GTGGTAT63750.021.456971
ACATGGG231450.020.380253
GAGTACT253350.020.37994812-13
TATAACG5850.020.2172742
AGAGTAC349050.019.84594310-11
CAGAGTA371600.019.77957210-11
TGGTATC65600.019.0692632
GTACTTT269800.019.00334214-15
Produced by FastQC (version 0.11.5)