FastQCFastQC Report
Thu 2 Jun 2016
DRR015223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7622512
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231130.30322025075198306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207020.27159025790972846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119810.15717915563793142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140550.051.4516331
GTATCAA252300.038.8253631
ATCAACG307650.031.2629323
TCAACGC308400.031.2312034
TATCAAC317700.030.4758532
CAACGCA317800.030.3525945
AACGCAG330950.029.2430386
GTGGTAT68300.027.722231
TGGTATC68550.026.5078962
ACGCAGA374750.025.6682667
CGCAGAG376500.025.3434078
GTACATG219450.024.033831
GCAGAGT406300.023.036429
TACATGG219600.022.2731592
ACATGGG222050.021.042113
GAGTACT280950.020.31902912-13
CAGAGTA398750.019.97897310-11
TACCTGG58750.019.7670632
AGAGTAC375000.019.52655610-11
GTACTTT303050.018.5042914-15
Produced by FastQC (version 0.11.5)