FastQCFastQC Report
Thu 2 Jun 2016
DRR015222_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015222_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11576498
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231100.19962859234286567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185900.16058396934893437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162350.050.4176751
GTATCAA306450.040.9356461
ATCAACG374300.033.056263
TCAACGC377600.032.728544
TATCAAC402400.030.9419562
CAACGCA400200.030.8670675
AACGCAG418450.029.6790146
ACGCAGA465600.026.1929997
CGCAGAG466800.025.9012818
GTACATG323900.023.89141
GTGGTAT79400.023.3746221
GCAGAGT506350.023.2404969
TACATGG326200.022.4110812
TGGTATC80750.021.2387892
ACATGGG338050.020.7091523
AGAGTAC469750.019.902310-11
GAGTACT320950.019.67307712-13
CAGAGTA498250.018.99204410-11
AGTACTT339950.018.88120312-13
GTACTTT350250.017.87172914-15
Produced by FastQC (version 0.11.5)