FastQCFastQC Report
Thu 2 Jun 2016
DRR015222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11576498
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263190.22734854703037136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218930.18911591398365896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133320.1151643614502417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180350.052.1455351
GTATCAA332200.040.1086921
ATCAACG406850.032.190533
TCAACGC409600.031.9299344
CAACGCA426550.030.6071665
TATCAAC432300.030.4361922
AACGCAG444450.029.4154456
ACGCAGA506550.025.6292257
CGCAGAG505250.025.5239898
GTACATG333800.024.0318831
TGGTATC85300.023.5994152
GTGGTAT91000.023.4671171
GCAGAGT557600.022.6215349
TACATGG328600.022.5223562
ACATGGG336350.021.1883163
GAGTACT364900.020.30910112-13
AGAGTAC515000.019.93626810-11
CAGAGTA547050.019.22176610-11
AGTACTT385050.019.0216912-13
GTACTTT404850.018.24232114-15
Produced by FastQC (version 0.11.5)