FastQCFastQC Report
Thu 2 Jun 2016
DRR015221_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015221_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5083124
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170580.33558103245169707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137690.27087672856298606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95250.18738476574641894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99050.050.2294121
TACCTGG47950.043.8320852
GTATCAA191500.041.792291
GTACCTG53300.040.2978131
GTACCGG4800.033.251761
ACCTGGG62750.032.841523
ATCAACG246150.031.9731223
TCAACGC249050.031.6528134
TATCAAC255750.030.9152582
CAACGCA260100.030.4814745
AACGCAG271950.029.2841916
TATAACG5650.028.9863032
ACAACGC11400.027.9339263
GTACCCG3650.026.2370031
GTGGTAT46350.026.1709651
TAACGCA7000.025.993354
ACGCAGA303850.025.7878517
GTACATG209650.025.5799851
CGCAGAG305200.025.5250288
TGGTATC46100.025.4599512
Produced by FastQC (version 0.11.5)