FastQCFastQC Report
Thu 2 Jun 2016
DRR015221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5083124
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195040.3837010468365517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156810.3084913923012698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108620.21368748824541756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109400.053.5595661
ACAACGC9000.041.498263
GTATCAA207000.040.850221
TACCTGG48900.039.4021572
GTACCTG53150.036.7476731
TATAACG5400.033.740752
GTGGTAT46550.031.5664351
ATCAACG267050.031.416343
ACCTGGG59900.031.0996153
TCAACGC272350.030.8049744
TATCAAC277550.030.3447932
TGGTATC47850.029.8907092
CAACGCA282800.029.8291385
AACGCAG298900.028.2528786
GTACCGG5150.026.5829621
ACGCAGA335900.024.9919367
CGCAGAG337700.024.7778388
TAACGCA7550.024.7340624
GTACAAC20200.023.720691
GTACATG218050.023.6907981
Produced by FastQC (version 0.11.5)