FastQCFastQC Report
Thu 2 Jun 2016
DRR015220_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015220_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7119653
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152670.21443460797878774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132890.18665235510775596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73730.10355841780491269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104100.051.160441
GTATCAA188200.039.6374631
ATCAACG228000.032.2594343
TCAACGC231350.031.7710844
TATCAAC242400.030.4377122
CAACGCA242050.030.384135
AACGCAG252150.029.2529496
ACGCAGA283050.025.7359687
CGCAGAG285300.025.373628
GTGGTAT47150.025.3373811
GTACATG188900.024.2833251
GCAGAGT305950.022.9922269
TACATGG193400.021.9911982
TGGTATC51700.021.8200072
ACATGGG198300.020.6902313
GAGTACT204250.019.70412-13
CAGAGTA300400.019.66337210-11
AGAGTAC282050.019.62870610-11
GAGTACA145150.018.4400961
GTACTTT219850.018.27514814-15
Produced by FastQC (version 0.11.5)