FastQCFastQC Report
Thu 2 Jun 2016
DRR015220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7119653
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173900.24425347696018335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154430.21690663856791897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88910.12487968163616962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117350.052.3867451
GTATCAA206200.038.3793261
ATCAACG248500.031.283893
TCAACGC251900.030.8980144
CAACGCA259800.029.9785585
TATCAAC265500.029.3855572
AACGCAG271650.028.788126
GTGGTAT51650.028.0991861
ACGCAGA313250.024.6894477
CGCAGAG313150.024.4792848
GTACATG194700.023.768891
TGGTATC57300.023.4481452
GCAGAGT335800.022.4350139
TACATGG196250.021.861622
ACATGGG197150.020.9981333
ACAACGC9050.020.1291313
GAGTACT234850.019.86752912-13
CAGAGTA333400.019.70866410-11
AGAGTAC314200.019.35558310-11
AGTACTT241700.018.56053712-13
Produced by FastQC (version 0.11.5)