FastQCFastQC Report
Thu 2 Jun 2016
DRR015219_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015219_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7474871
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164220.21969609910324875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140590.188083513414479No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80610.10784132595733091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120850.050.228321
GTATCAA221800.039.7865871
ATCAACG268350.032.1534163
TCAACGC271350.031.897014
TATCAAC281500.030.8138052
CAACGCA284100.030.4634785
AACGCAG296550.029.4417026
GTACATG243800.025.4216081
ACGCAGA339300.025.382227
CGCAGAG338450.025.3116138
TACATGG247800.024.1010762
GTGGTAT57550.024.0904831
GCAGAGT366200.022.8470469
ACATGGG260300.022.2266673
TGGTATC58950.021.8361282
GTACCGG7450.020.8132511
TACCTGG57050.020.0120282
AGAGTAC335500.019.53650710-11
GAGTACT226000.019.23471812-13
CAGAGTA359700.019.14492210-11
Produced by FastQC (version 0.11.5)