FastQCFastQC Report
Thu 2 Jun 2016
DRR015219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7474871
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194420.26009813413502386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170810.22851230476084472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103710.1387448693094503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135750.053.144761
GTATCAA244400.039.134381
ATCAACG299500.031.4929283
TCAACGC301350.031.3147034
TATCAAC311100.030.4073032
CAACGCA312750.030.2037965
AACGCAG327300.028.9306436
ACGCAGA372700.025.1866267
CGCAGAG373600.024.9431728
GTACATG248900.024.4017831
GTGGTAT62950.023.6314431
TACATGG249650.023.297042
TGGTATC61450.022.5316542
GCAGAGT406550.022.4176889
ACATGGG257500.021.454253
GAGTACT264200.019.79856712-13
AGAGTAC372650.019.55218310-11
CAGAGTA399900.019.54038610-11
TACCTGG60250.019.5031192
CCGGTCG7200.018.9683489
Produced by FastQC (version 0.11.5)