FastQCFastQC Report
Thu 2 Jun 2016
DRR015218_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015218_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5122851
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247170.4824852411284264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212610.41502280663638275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135270.26405218500401434No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55920.10915796692115387No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51680.10088132565245407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134850.052.0153661
GTATCAA236100.038.710431
TATAACG5500.033.9105032
ATCAACG303300.029.980043
TCAACGC305100.029.6976454
TATCAAC306450.029.6733252
TAACGCA6250.029.1108464
GTGGTAT54000.028.7151761
CAACGCA320850.028.2654275
AACGCAG332700.027.4446986
GTACATG259400.025.5811041
TACATGG260550.024.8617632
TGGTATC60750.024.6355722
ACGCAGA368750.024.451917
CGCAGAG373550.024.076858
TACCTGG44100.022.7964252
ACATGGG273400.022.6106363
GCAGAGT388000.022.4416589
GAGTACT242900.020.1532312-13
CAGAGTA379050.020.02111610-11
Produced by FastQC (version 0.11.5)