FastQCFastQC Report
Thu 2 Jun 2016
DRR015218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5122851
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300240.5860798996496287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250970.489902985661695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165790.3236283858343723No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67910.13256290296165163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142000.053.5142821
GTATCAA261450.041.3891681
ATCAACG340250.031.5208233
TATCAAC345850.031.1338182
TCAACGC346200.031.0323124
CAACGCA358650.030.0965655
AACGCAG370350.029.2563936
GTGGTAT58950.028.7232741
TATAACG4950.027.6050622
ACGCAGA413550.026.0466237
CGCAGAG417850.025.6369428
TGGTATC64950.025.1059442
GCAGAGT437200.024.0232729
TAACGCA6250.023.3175664
GTACATG264000.023.1137981
TACATGG265750.022.4013922
TACCTGG43350.021.644662
ACATGGG272100.021.0550773
AGAGTAC414050.020.78684610-11
GAGTACT282850.020.72314812-13
Produced by FastQC (version 0.11.5)