FastQCFastQC Report
Thu 2 Jun 2016
DRR015217_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015217_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7920939
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212930.2688191387410003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180110.22738465729883794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107090.13519861723464857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157400.045.6915741
GTATCAA276450.037.150181
ATCAACG337200.030.113843
TCAACGC339950.029.8415814
TATCAAC354600.028.7782062
CAACGCA359350.028.2405285
AACGCAG377350.026.9855986
GTACATG299150.026.388651
TACATGG296000.025.3148372
ACGCAGA418750.023.9135277
CGCAGAG419300.023.6869378
ACATGGG309850.023.2134593
GCAGAGT446800.021.6994979
GTGGTAT78650.020.8743481
GAGTACT266950.019.2857912-13
AGAGTAC399200.019.2583710-11
TACCTGG58300.019.0412062
CATGGGG178900.018.689994
CAGAGTA438900.018.49556210-11
GTACTTT274800.018.38731214-15
Produced by FastQC (version 0.11.5)