FastQCFastQC Report
Thu 2 Jun 2016
DRR015217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7920939
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221120.27915882195280134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190090.23998417359356006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119150.1504240848212567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169400.046.870921
GTATCAA285600.035.949421
ATCAACG353750.028.7075863
TCAACGC357250.028.4263364
TATCAAC369100.027.652572
CAACGCA370700.027.371425
AACGCAG387450.026.3525986
GTACATG309900.023.9423371
TACATGG303600.023.2673912
ACGCAGA438300.023.0155777
CGCAGAG439900.022.7145488
GTGGTAT79650.022.6297051
ACATGGG311000.021.8765323
GCAGAGT475100.020.7346089
TACCTGG58600.020.2852572
GAGTACT274900.019.56487712-13
AGAGTAC423100.019.24811410-11
TGGTATC88550.019.184832
AGTACTT283800.018.43803612-13
CAGAGTA465950.018.2986610-11
Produced by FastQC (version 0.11.5)