FastQCFastQC Report
Thu 2 Jun 2016
DRR015216_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015216_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5543267
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169490.3057583190562533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144050.2598648053575626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82770.14931627864939576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102000.049.5684361
GTATCAA176850.037.405561
ATCAACG212350.030.7001953
TCAACGC214400.030.4253984
TATCAAC224550.029.2556112
CAACGCA224000.029.056335
AACGCAG235300.027.8306316
GTACATG155000.026.2071531
TACCTGG44950.024.9989812
CGCAGAG261000.024.8616948
ACGCAGA262200.024.817287
TACATGG154600.024.483242
GTGGTAT51900.024.244631
GTACCTG48650.023.7089081
TGGTATC51150.023.0361122
GCAGAGT276850.022.7975529
ACATGGG160950.022.7254413
GAGTACT193450.020.3216612-13
GTACCGG5200.020.1650051
CAGAGTA272900.019.82029510-11
Produced by FastQC (version 0.11.5)