FastQCFastQC Report
Thu 2 Jun 2016
DRR015216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5543267
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203700.36747282784682755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175840.3172136575777425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104510.1885350281702108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115000.051.0761451
GTATCAA195450.037.174471
ATCAACG238550.029.8211253
TCAACGC239950.029.7040754
CAACGCA248850.028.8106865
TATCAAC249250.028.5985982
AACGCAG259550.027.7632666
ACAACGC8400.027.651163
TACCTGG46800.025.8881722
GTACCTG48200.025.7548621
GTGGTAT56200.025.5806541
GTACATG160350.025.1605821
ACGCAGA292950.024.4422957
TGGTATC55550.024.2701932
CGCAGAG292950.024.2247548
TACATGG159850.023.9349352
ACATGGG163400.022.7994143
GCAGAGT311500.022.636029
TCTGTCG13750.021.1877128
GAGTACT228200.020.55630112-13
Produced by FastQC (version 0.11.5)