FastQCFastQC Report
Thu 2 Jun 2016
DRR015215_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015215_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5997094
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180430.3008623843481526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153560.25605735044339806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93410.1557587724988136No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112600.049.4579351
GTATCAA204400.038.621891
ATCAACG250800.031.2761273
TCAACGC254200.030.8388564
TATCAAC259200.030.4055982
CAACGCA264100.029.7665395
AACGCAG275950.028.6333966
TACCTGG54200.028.560852
GTGGTAT54450.026.7647691
GTACCTG60450.026.1487221
ACGCAGA303650.025.7512787
TATAACG6200.025.7020552
CGCAGAG304100.025.6227788
TGGTATC54950.024.6910762
GTACATG170300.023.580061
GCAGAGT323200.023.5174589
TAACGCA7900.022.4738884
TACATGG169950.022.2891562
GTACCGG6800.021.4986781
ATAACGC7500.021.2452563
Produced by FastQC (version 0.11.5)